Mouse Monoclonal Antibody to HDAC1

Item Information

Catalog #

Size

Price

Description

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Product Overview

Entrez GenelD

3065

Aliases

HD1; RPD3; KDAC1; GON-10; RPD3L1

Clone#

1B6A7

Host / Isotype

Mouse / IgG1

Immunogen

Purified recombinant fragment of human HDAC1 (AA: 321-482) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Cell Commun Signal. 2019 Jul 29;17(1):86. 2.Hum Pathol. 2019 Mar;85:194-201.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using HDAC1 mAb against human HDAC1 (AA: 321-482) recombinant protein. (Expected MW is 44.6 kDa)

Western Blot

Figure 3:Western blot analysis using HDAC1 mAb against HEK293-6e (1) and HDAC1 (AA: 1-482)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using HDAC1 mouse mAb against NIH/3T3 (1), Hela (2), Raw264.7 (3), K562 (4), Jurkat (5), C6 (6), and Raji (7) cell lysate.

Flow cytometric analysis