Mouse Monoclonal Antibody to GOLGA2
Item Information
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Description
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.
Product Overview
Entrez GenelD
2801
Aliases
GM130
Clone#
2D3D12
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human GOLGA2 (AA: 1-205) expressed in HEK293-6e cells supernatant.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/50 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Sci Rep. 2015 Aug 28;5:13324. 2.Cell Cycle. 2015;14(8):1139-47.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using GOLGA2 mAb against human GOLGA2 (AA: 1-205) recombinant protein. (Expected MW is 52.7 kDa)
Western Blot
Figure 3:Western blot analysis using GOLGA2 mouse mAb against HepG2 (1), Hela (2), K562 (3), and HEK293 (4) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using GOLGA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Hela cells using GOLGA2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using GOLGA2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using GOLGA2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.