GLUL Primary Antibody
Item Information
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Price
Description
The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
2752
Aliases
GS; GLNS; PIG43; PIG59
Clone#
2D11A2
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human GLUL (AA: 2-121) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1,J Proteome Res. 2019 Mar 1;18(3):1352-1362;2,J Cell Biochem. 2018 Jul;119(7):6008-6015.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using GLUL mAb against human GLUL (AA: 2-121) recombinant protein. (Expected MW is 30.7 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using GLUL mAb against HEK293-6e (1) and GLUL (AA: 2-121)-hIgGFc transfected HEK293-6e (2) cell lysate.
WESTERN BLOT
Figure 4: Western blot analysis using GLUL mouse mAb against Jurkat (1), and mouse liver (2) cell lysate.
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of Hela cells using GLUL mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY
Figure 6: Immunohistochemical analysis of paraffin-embedded cerebellar tissues using GLUL mouse mAb with DAB staining.
IMMUNOFLUORESCENCE ANALYSIS
Figure 7: Immunofluorescence analysis of hela cells using GLUL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.