Mouse Monoclonal Antibody to CXCL9

Item Information
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Description

This antimicrobial gene is part of a chemokine superfamily that encodes secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded is thought to be involved in T cell trafficking. The encoded protein binds to C-X-C motif chemokine 3 and is a chemoattractant for lymphocytes but not for neutrophils. [provided by RefSeq, Aug 2020]

Product Overview
Entrez GenelD
4283
Aliases
CMK; MIG; Humig; SCYB9; crg-10
Clone#
7E4A10
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human CXCL9 (AA: 23-125) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Review Adv Exp Med Biol. 2020;1231:45-51. 2,bioRxiv. 2020 Jun 22;2020.06.22.165225.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CXCL9 mAb against human CXCL9 (AA: 23-125) recombinant protein. (Expected MW is 37.7kDa)
Figure 2:Western blot analysis using CXCL9 mAb against human CXCL9 (AA: 23-125) recombinant protein. (Expected MW is 37.7kDa)
Western Blot
Figure 3:Western blot analysis using CXCL9 mAb against HEK293-6e (1) and CXCL9 (AA: 23-125)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using CXCL9 mAb against HEK293-6e (1) and CXCL9 (AA: 23-125)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of K562 cells using CXCL9 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of K562 cells using CXCL9 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of THP-1 cells using CXCL9 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of THP-1 cells using CXCL9 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.