Mouse Monoclonal Antibody to CX3CR1
Item Information
Catalog #
Size
Price
Description
Fractalkine is a transmembrane protein and chemokine involved in the adhesion and migration of leukocytes. The protein encoded by this gene is a receptor for fractalkine. The encoded protein also is a coreceptor for HIV-1, and some variations in this gene lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Four transcript variants encoding two different isoforms have been found for this gene.
Product Overview
Entrez GenelD
1524
Aliases
V28; CCRL1; GPR13; CMKDR1; GPRV28; CMKBRL1
Clone#
2H4C9
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human CX3CR1 (AA: extra mix) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Curr Pharm Biotechnol. 2018;19(11):869-876. 2,Psychiatry Clin Neurosci. 2018 Jun;72(6):409-422.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CX3CR1 mAb against human CX3CR1 (AA: extra mix) recombinant protein. (Expected MW is 24 kDa)
Western Blot
Figure 3:Western blot analysis using CX3CR1 mAb against HEK293-6e (1) and CX3CR1 (AA: extra mix)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Jurkat cells using CX3CR1 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of THP-1 cells using CX3CR1 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Immunofluorescence analysis of Hela cells using CX3CR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.