Mouse Monoclonal Antibody to CD79A
Item Information
Catalog #
Size
Price
Description
The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Product Overview
Entrez GenelD
973
Aliases
IGA; MB-1
Clone#
4A10D12
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human CD79A (AA: extra(33-143)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Korean J Lab Med. 2007 Dec;27(6):388-93. 2,Cancer Genet Cytogenet. 2007 Apr 1;174(1):76-7.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD79A mAb against human CD79A (AA: extra(33-143)) recombinant protein. (Expected MW is 28.3kDa)
Western Blot
Figure 3:Western blot analysis using *** mouse mAb against Raji (1), Ramos (2), k562 (3), and MOLT4 (4) cell lysate.
Western Blot
Figure 4:Western blot analysis using CD79A mAb against HEK293-6e (1) and CD79A (AA:extra(33-143))-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Raji cells using CD79A mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Immunofluorescence analysis of Hela cells using CD79A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.