CD3D Primary Antibody
Item Information
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Price
Description
The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]
Product Overview
Entrez GenelD
915
Aliases
T3D; IMD19; CD3-DELTA
Clone#
6B6B12
Host / Isotype
Mouse / Mouse IgG2b
Immunogen
Purified recombinant fragment of human CD3D (AA: 127-171) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1,Case Reports J Clin Invest. 2011 Oct;121(10):3872-6;2,Case Reports Can Respir J. Jan-Feb 2014;21(1):17-9;3,Cytometry B Clin Cytom. 2009 May;76(3):213-7.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using CD3D mAb against human CD3D (AA: 127-171) recombinant protein. (Expected MW is 18.6 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using CD3D mAb against HEK293-6e (1) and CD3D (AA: 127-171))-hIgGFc transfected HEK293-6e (2) cell lysate.
FLOW CYTOMETRY
Figure 4: Flow cytometric analysis of Jurkat cells using CD3D mouse mAb (green) and negative control (red).
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of THP-1 cells using CD3D mouse mAb (green) and negative control (red).
IMMUNOFLUORESCENCE ANALYSIS
Figure 6: Immunofluorescence analysis of Hela cells using CD3D mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.