Mouse Monoclonal Antibody to CD34

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.

Product Overview

Entrez GenelD

947

Aliases

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Clone#

4A3E7

Host / Isotype

Mouse / IgG1

Immunogen

Purified recombinant fragment of human CD34 (AA: extra 32-290) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/20 - 1/100

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Stem Cell Res Ther. 2019 May 21;10(1):147. 2.Circ Res. 2017 Apr 28;120(9):1466-1476.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CD34 mAb against human CD34 (AA: extra 32-290) recombinant protein. (Expected MW is 42.3 kDa)

Western Blot

Figure 3:Western blot analysis using CD34 mAb against HEK293 (1) and CD34 (AA: extra 32-290)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using CD34 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis