CD314 Primary Antibody

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Description

Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. This gene encodes a member of the NKG2 family. The encoded transmembrane protein is characterized by a type II membrane orientation (has an extracellular C terminus) and the presence of a C-type lectin domain. It binds to a diverse family of ligands that include MHC class I chain-related A and B proteins and UL-16 binding proteins, where ligand-receptor interactions can result in the activation of NK and T cells. The surface expression of these ligands is important for the recognition of stressed cells by the immune system, and thus this protein and its ligands are therapeutic targets for the treatment of immune diseases and cancers. Read-through transcription exists between this gene and the upstream KLRC4 (killer cell lectin-like receptor subfamily C, member 4) family member in the same cluster.

Product Overview
Entrez GenelD
22914
Aliases
KLRK1; KLR; NKG2D; NKG2-D; D12S2489E
Clone#
3A7A2
Host / Isotype
Mouse / Mouse IgG2a
Immunogen
Purified recombinant fragment of human CD314 (AA: extra 73-216) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Mol Immunol. 2019 Feb;106:22-28. 2.Front Immunol. 2018 Mar 8;9:441.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using CD314 mAb against human CD314 (AA: extra 73-216) recombinant protein. (Expected MW is 42.6 kDa)
Figure 2: Western blot analysis using CD314 mAb against human CD314 (AA: extra 73-216) recombinant protein. (Expected MW is 42.6 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using CD314 mAb against HEK293-6e (1) and CD314 (AA: extra 73-216)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3: Western blot analysis using CD314 mAb against HEK293-6e (1) and CD314 (AA: extra 73-216)-hIgGFc transfected HEK293-6e (2) cell lysate.
WESTERN BLOT
Figure 4: Western blot analysis using CD314 mouse mAb against Rat Spleen (1), A549 (2), and HepG2 (3) cell lysate.
Figure 4: Western blot analysis using CD314 mouse mAb against Rat Spleen (1), A549 (2), and HepG2 (3) cell lysate.
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of Jurkat cells using CD314 mouse mAb (green) and negative control (red).
Figure 5: Flow cytometric analysis of Jurkat cells using CD314 mouse mAb (green) and negative control (red).
FLOW CYTOMETRY
Figure 6: Flow cytometric analysis of THP-1 cells using CD314 mouse mAb (green) and negative control (red).
Figure 6: Flow cytometric analysis of THP-1 cells using CD314 mouse mAb (green) and negative control (red).
FLOW CYTOMETRY
Figure 7: Flow cytometric analysis of U937 cells using CD314 mouse mAb (green) and negative control (red).
Figure 7: Flow cytometric analysis of U937 cells using CD314 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.

Catalog#: 31917

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