Mouse Monoclonal Antibody to CD109

Item Information

Catalog #

Size

Price

Description

This gene encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene.

Product Overview

Entrez GenelD

135228

Aliases

p180; r150; CPAMD7

Clone#

1A3D3

Host / Isotype

Mouse / IgG1

Immunogen

Purified recombinant fragment of human CD109 (AA: extra 1274-1421) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/20 - 1/100

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Cancer Sci. 2020 May;111(5):1652-1662. 2.Diagn Pathol. 2015 Aug 7;10:137.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CD109 mAb against human CD109 (AA: extra 1274-1421) recombinant protein. (Expected MW is 42.3 kDa)

Western Blot

Figure 3:Western blot analysis using CD109 mAb against HEK293-6e (1) and CD109 (AA: extra 1274-1421)-hIgGFc transfected HEK293-6e (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using CD109 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis