Mouse Monoclonal Antibody to CCR10

Item Information

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Description

Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC, based on the arrangement of the first 2 of the 4 conserved cysteine residues; the 2 cysteines are separated by a single amino acid in CXC chemokines and are adjacent in CC chemokines. CCR10 is the receptor for CCL27 (SCYA27; MIM 604833); CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation (Homey et al., 2002 [PubMed 11821900]).[supplied by OMIM, Mar 2008]

Product Overview

Entrez GenelD

2826

Aliases

GPR2

Clone#

6E3E4

Host / Isotype

Mouse / IgG1

Immunogen

Purified recombinant fragment of human CCR10 (AA: extra mix) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1,Allergy. 2019 May;74(5):933-943. 2,Int Immunopharmacol. 2017 Oct;51:124-130.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CCR10 mAb against human CCR10 (AA: extra mix) recombinant protein. (Expected MW is 38 kDa)

Western Blot

Figure 3:Western blot analysis using CCR10 mAb against HEK293-6e (1) and CCR10 (AA: extra mix)-hIgGFc transfected HEK293-6e (2) cell lysate.

Flow cytometric analysis

Figure 4:Flow cytometric analysis of HL-60 cells using CCR10 mouse mAb (green) and negative control (red).

Flow cytometric analysis

Figure 5:Flow cytometric analysis of MOLT4 cells using CCR10 mouse mAb (green) and negative control (red).

Flow cytometric analysis

Figure 6:Flow cytometric analysis of THP-1 cells using CCR10 mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CCR10 mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 8:Immunofluorescence analysis of Hela cells using CCR10 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

For Research Use Only. Not for use in diagnostic procedures.