Mouse Monoclonal Antibody to BCL2

Item Information

Catalog #

Size

Price

Description

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Product Overview

Entrez GenelD

596

Aliases

Bcl-2; PPP1R50

Clone#

3B5H7

Host / Isotype

Mouse / IgG1

Immunogen

Purified recombinant fragment of human BCL2 (AA: 1-239) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Nucleic Acids Res. 2020 Dec 16;48(22):12727-12745. 2.Prog Transplant. 2020 Jun;30(2):184-186.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using BCL2 mAb against human BCL2 (AA: 1-239) recombinant protein. (Expected MW is 29.2 kDa)

Western Blot

Figure 3:Western blot analysis using BCL2 mAb against HEK293-6e (1) and BCL2 (AA: 1-239)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using BCL2 mouse mAb against Raji (1), MCF-7 (2), Jurkat (3), A549 (4), HEK293 (5), T47D (6), Hela (7), and C6 (8) cell lysate.

Flow cytometric analysis