4E-BP1 Primary Antibody

Item Information
Catalog # Size/Concentration Price
20105 100μl $264.00
Specification
AliasesBP-1; 4EBP1; 4E-BP1; PHAS-I; MGC4316; EIF4EBP1
Product OrderE
Clone#11G12C11
Entrez GeneID1978
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of 4EBP1 expressed in E. Coli.
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C.  Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P (Immunohistochemistry) 1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
N/A
Catalog
20105
$264.00
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Images

Western Blot

Cell Culture Products
Figure 1: Western blot analysis using 4E-BP1 mouse mAb against truncated 4E-BP1 recombinant protein (1).

Immunohistochemical analysis

Cell Culture Products
Figure 2: Immunohistochemical analysis of paraffin-embedded human pancreas carcinoma (A), esophagus carcinoma tissue (B) and ovary tumor tissue, showing cytoplasmic and membrane localization using 4E-BP1 mouse mAb with DAB staining.
Product Overview
Description 4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.
References (references)
References (references) 1. Pause, A. et al. 1994.Nature. 371:762-767.
2. Fadden, P. et al. 1997. J. Biol. Chem. 272:10240-10247.

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