NanoFect™ Transfection Reagent is a unique blend of polymers that delivers more DNA and siRNAs to cells than the leading lipid-based transfection kits. NanoFect™ is a powerful, broadly applicable transfection reagent for effective and reproducible transfections. The NanoFect reagent self-assembles nanoparticles in the presence of DNA and RNA. These complexes are readily taken up by target cells for efficient gene delivery. No media changes are required as NanoFect works in the presence of antibiotics and serum. The easy-to-use protocol with rapid, one-step incubation for 15 minutes before adding directly to target cells makes NanoFect well-suited for high-throughput transfection experiments.