EpCAM CAR-T cells (EpCAM scFv-TM-CD28-CD3z)

EpCAM (epithelial cell adhesion molecule, CD326) is a type I membrane protein that mediates calcium-independent epithelial cell-cell adhesion. EpCAM is cleaved into an extracellular domain and an intracellular domain, the latter shuttling to the nucleus and inducing the transcription of various genes, including the oncogene c-myc which promotes tumor growth. EpCAM is overexpressed in many epithelial cell cancers and is also expressed in cancer stem cells, making EpCAM an attractive target for immunotherapy.

ProMab's PM-CAR1019 primary human CAR-T cells express an anti-EpCAM CAR containing an EpCAM-specific scFv, hinge and transmembrane domains, the CD28 costimulatory domain and the CD3zeta activation domain. The CAR-T cells are generated by transducing activated human T cells with replication-defective lentivirus encoding the CAR. After expansion, the cells are cryopreserved and shipped in vials containing up to 10 million cells. For orders over 10 million cells, please inquire. All ProMab cells are guaranteed to be sterile, pathogen-free and mycoplasma-free.