CD47scFv-TM28-CD28-CD3z

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Description

ProMab's PM-CAR1040 primary human CAR-T cells express an anti-CD47 CAR containing a CD47-specific scFv, hinge and transmembrane domains, the CD28 costimulatory domain and the CD3zeta activation domain.

 

Product Overview
Cell Type Tested
T cells
Formulation
CS10 CryoStor Medium (Sigma Aldrich, Cat, #C2874)
Cell Viability
>90%
Mycoplasma Testing
The cells has been screened using a PCR-based mycoplasma detection kit to conirm the absence of mycoplasma species.
Storage
-80°C; liquid nitrogen
Shipping & Handing
This product will be shipped in a box containing dry ice at a temperature of -70°C.
Product Image
CAR Structure
Figure 1. Schematic representation of the scFv, costimulatory domain, and activation domain of PM-CAR1040. This construct targets CD47. 
Figure 1. Schematic representation of the scFv, costimulatory domain, and activation domain of PM-CAR1040. This construct targets CD47. 
RTCA
Figure 2. RTCA cytotoxicity activity of effector CD47-CD28-CD3-CAR-T cells against SKOV-3 ovarian cancer target cells. Effector cells: Target cells ratio=10:1.
Figure 2. RTCA cytotoxicity activity of effector CD47-CD28-CD3-CAR-T cells against SKOV-3 ovarian cancer target cells. Effector cells: Target cells ratio=10:1.
IL-2 secretion
Figure 3. IL-2 secretion by CD47-CAR-T cells against different target cancer cell lines.
Figure 3. IL-2 secretion by CD47-CAR-T cells against different target cancer cell lines.
For Research Use Only. Not for use in diagnostic procedures.