CD4scFv-FLAG-TM28-CD28-CD3z

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Description

CD4scFv-FLAG-TM28-CD28-CD3z CAR-T cells are generated with a lentivirus expressing CAR from normal human healthy donors. All cells are available at 1x10^6 to 1x10^7 cells in frozen vials, and shipped on dry-ice. For orders larger than >1x10^9, please inquire. CAR-T cells are cryopreserved in next-generation cryopreservation CS10 CryoStor medium (Sigma Aldrich, Catalog Number: C2874). All our cells are pathogen- and mycoplasma- free.

Product Overview
Cell Type Tested
T cells
Formulation
CS10 CryoStor Medium (Sigma Aldrich, Cat, #C2874)
Cell Viability
>90%
Mycoplasma Testing
The cells has been screened using a PCR-based mycoplasma detection kit to conirm the absence of mycoplasma species.
Storage
-80°C; liquid nitrogen
Shipping & Handing
This product will be shipped in a box containing dry ice at a temperature of -70°C.
Product Image
CAR Structure
Figure 1. Schematic representation of the scFv, costimulatory domain, and activation domain of PM-CAR1032. This construct targets CD4. 
Figure 1. Schematic representation of the scFv, costimulatory domain, and activation domain of PM-CAR1032. This construct targets CD4. 
FACS
Figure 2. Beam1 Ab detects CD4-Beam 1TM-CAR-T cells after transduction with lentiviral CD4-Beam1-CAR construct. FACS data is shown.
Figure 2. Beam1 Ab detects CD4-Beam 1TM-CAR-T cells after transduction with lentiviral CD4-Beam1-CAR construct. FACS data is shown.
LDH Assay
Figure 3. CD4-Beam1TM-CD28-CD3-CAR-T cells kill CEM leukemia target cells (positive for CD4). Effector: Target cells ratio=10:1.
Figure 3. CD4-Beam1TM-CD28-CD3-CAR-T cells kill CEM leukemia target cells (positive for CD4). Effector: Target cells ratio=10:1.
For Research Use Only. Not for use in diagnostic procedures.