CD22scFv-TF-TM28-CD28-CD3z
Item Information
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Description
CD22scFv-TF-TM28-CD28-CD3z CAR-T cells are generated with a lentivirus expressing CAR from normal human healthy donors. All cells are available at 1x10^6 to 1x10^7 cells in frozen vials, and shipped on dry-ice. For orders larger than >1x10^9, please inquire. CAR-T cells are cryopreserved in next-generation cryopreservation CS10 CryoStor medium (Sigma Aldrich, Catalog Number: C2874). All our cells are pathogen- and mycoplasma- free.
Product Overview
Cell Type Tested
T cells
Formulation
CS10 CryoStor Medium (Sigma Aldrich, Cat, #C2874)
Cell Viability
>90%
Mycoplasma Testing
The cells has been screened using a PCR-based mycoplasma detection kit to conirm the absence of mycoplasma species.
Storage
-80°C; liquid nitrogen
Shipping & Handing
This product will be shipped in a box containing dry ice at a temperature of -70°C.
Product Image
CAR Structure
Figure 1. Schematic representation of the scFv, costimulatory domain, and activation domain of PM-CAR1047. This construct targets CD22.
FACS
Figure 2. FACS analysis with Beam-2 antibody shows expression of CD22-CAR.
RTCA
Figure 3. RTCA cytotoxicity activity of effector CD22-Beam-2 CD28-CD3-CAR-T cells against CHO-CD22 target cells available from ProMab. CD22 -CAR-T cells are also shown. Effector: Target cells ratio=10:1 (Top panel). RTCA cytotoxicity activity of effector CD22-Beam-2-CD28-CD3-CAR-T cells against CHO cells.
For Research Use Only. Not for use in diagnostic procedures.