CD19 CAR-T cells (CD19 scFv-FLAG-TM-41BB-CD3z /// tEGFR)

CD19 is a type I membrane protein expressed by all B cells and B cell precursors but not by plasma cells or other cell types. CD19 functions as part of the B cell antigen receptor complex, allowing B cells to respond to foreign antigens. CD19 is also expressed on all B cell malignancies and is a favorable target for immunotherapy because of its specificity; patients receive immunoglobulin injections because their own B cells are depleted along with the malignant cells.

ProMab's PM-CAR1067 primary human CAR-T cells express an anti-CD19 CAR containing a CD19-specific scFv, a FLAG tag, hinge and transmembrane domains, the 4-1BB costimulatory domain and the CD3zeta activation domain. In addition, PM-CAR1067 CAR-T cells express truncated EGFR, driven by a separate promoter, for Cetuximab-mediated deletion by ADCC. The CAR-T cells are generated by transducing activated human T cells with replication-defective lentivirus encoding the CAR. After expansion, the cells are cryopreserved and shipped in vials containing up to 10 million cells. Please inquire for orders over 10 million cells. All ProMab cells are guaranteed to be sterile, pathogen-free and mycoplasma-free.