CD19scFv-CD22scFv-TM-4-1BB-CD3z-T2A-RQR8
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Description
CD19scFv-CD22scFv-TM-4-1BB-CD3z-T2A-RQR8 CAR-T cells are generated with a lentivirus expressing CAR from normal human healthy donors. All cells are available at 1x10^6 to 1x10^7 cells in frozen vials, and shipped on dry-ice. For orders larger than >1x10^9, please inquire. CAR-T cells are cryopreserved in next-generation cryopreservation CS10 CryoStor medium (Sigma Aldrich, Catalog Number: C2874). All our cells are pathogen- and mycoplasma- free.
Product Overview
Cell Type Tested
T cells
Formulation
CS10 CryoStor Medium (Sigma Aldrich, Cat, #C2874)
Cell Viability
>90%
Mycoplasma Testing
The cells has been screened using a PCR-based mycoplasma detection kit to conirm the absence of mycoplasma species.
Storage
-80°C; liquid nitrogen
Shipping & Handing
This product will be shipped in a box containing dry ice at a temperature of -70°C.
Product Image
CAR Structure
Figure 1. Schematic representation of the scFv, costimulatory domain, and activation domain of PM-CAR1050. This construct targets CD19+CD22.
FACS
Figure 2. Rituximab detects Rituximab (R) epitope. CD34 antibody detected CD34 epitope (Q) (not shown).
RTCA
Figure 3 . CD19-CD22-41BB-CD3-T2A-RQR8 CAR-T cells kill CHO-Hela-CD19 cells. CD19-CD22-CAR-T cells with no suicide switch (PMC250 / PM-CAR1035, available from ProMab) are also shown.
RTCA
Figure 4. CD19-CD22-41BB-CD3-T2A-RQR8 CAR-T cells kill CHO-CD22 cells. CD19-CD22-CAR-T cells with no suicide switch (PMC250 / PM-CAR1035, available from ProMab) are also shown.
For Research Use Only. Not for use in diagnostic procedures.