ProMab Biotechnologies dedicated itself from the first day to develop monoclonal antibodies almost exclusively. We offer a completely human Fab library,constituting sequences derived from the antibody repertoire of approximately 120 individuals, with a diversity/complexity of approximately 1 x 10¹¹ clones. Or we can humanize non-human antibodies (antibodies raised, for example, in a mouse or rat host towards a human target molecule) , requiring the modification of the complementarity-determining regions (CDRs) to increase their similarity to human antibody variants.
Included in Human Library Display Package
- Fab library panning against provided antigen
- ELISA screening of Fab expressed in E.coli against antigen
- Sequencing of selected positive clones
- Heavy and light chain cloning into mammalian expression vector
- Transient transfection of HEK293 with recombinant plasmids
- ELISA and western blot confirmation of HEK293 supernatant containing human antibodies
- Delivery of clone sequences of five positive clones via an email message
Antibodies required for therapeutic use will be required to be structurally indistinct to other endogenous circulating human antibodies, to minimize immunogenicity following their introduction into the patient.
Antibody Humanization
A simple “copy-and-paste” approach to introduce a rodent CDR into a human antibody scaffold will often diminish or destroy the binding specificity and affinity of the original rodent antibody. Humanization therefore will rely on discrete CDR residue substitutions to obtain an optimum balance of minimal immunogenicity toward the antibody, while maintaining the original binding and recognition characteristics.
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