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CD70 (Cluster of Differentiation 70), encoded by the CD70 gene, is a cytokine belonging to the tumor necrosis factor (TNF) ligand family. This cytokine is the surface antigen and ligand of TNFRSF27/CD27. It is primarily expressed on highly activated T and B cells, as well as NK cells and mature dendritic cells. CD70 expression on T and B cells is stimulated by triggering T and B cell receptors and can be upregulated by several cytokines such as IL-1α, IL-2, IL-12, GM-CSF and TNF-α. As a co-stimulatory molecule, CD70 plays an important role in regulating immune system activation, in particular by improving the activation, proliferation and survival of T and B cells, resulting in a more effective immune response. CD70 has been shown to be overexpressed in several types of cancer, including Hodgkin's and non-Hodgkin's lymphomas, as well as certain types of solid tumors. Overexpression of CD70 in cancer cells correlates with cell proliferation and survival, and inhibits apoptosis, in cancer progression. Therefore, CD70 is considered as a possible target for the treatment of CD70-positive lymphomas. This product is designed as a tool for the delivery and expression of anti-CD70 CAR mRNA for research. The product leverages the lipid nanoparticle (LNP) technology platform for simple and efficient delivery of anti-CD70 CAR mRNA to a variety of mammalian cells in vitro and in vivo. The LNPs used are formulated with SM-102, DSPC, cholesterol and DMG-PEG2000 at an optimal molar concentration for a high rate of encapsulation and efficient mRNA delivery. The anti-CD70 CAR in this product is approximately 56 kD and consists of anti-CD70 scFv (single-chain variable fragment) linked to a second-generation CAR (chimeric antigen receptor) containing the CD8 hinge and transmembrane domain and 4-1BB and CD3ζ signaling domains. The full-length amino acid sequence of this anti-CD70 CAR mRNA-LNP product is available upon request.

Product Overview
mRNA-LNPs suspended in PBS (-Ca, -Mg) (pH: 7.0-7.4).
Cell Type Tested
Application & Handing
Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container. It is important not to spin for too long as this may rupture mRNA-LNPs. Do not vortex. Work with mRNA-LNPs on ice and minimize the time that the product spends at room temperature. After handling the product during experiments, return immediately to ice. mRNA-LNP products should only be handled with certified RNase-free reagents and consumables. Use of filtered pipette tips is highly recommended.
4°C; ice
For Research Use Only. Not for use in diagnostic procedures.