Vimentin (PT0495R) PT® Rabbit mAb

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Description

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009],

Product Overview
Entrez GenelD
7431
Clone#
PT0495R
Host / Isotype
Rabbit / IgG, Kappa
Species Reactivity
Human, Mouse, Rat
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage
-15°C to -25°C / 1 year (Do not store below -25°C)
Product Applications
WB (Western Blot)
1/1000 - 1/5000
IHC_P(Immunohistochemistry)
1/1000 - 1/5000
ELISA
1/5000 - 1/20000
Product Image
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Vimentin (PT0495R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Lane 1: Hela
Lane 2: U2OS
Lane 3: C6
Lane 4: U-14

Predicted band size: 54kDa
Observed band size: 54kDa
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Vimentin (PT0495R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: U2OS Lane 3: C6 Lane 4: U-14 Predicted band size: 54kDa Observed band size: 54kDa
Rat colon was stained with anti-Vimentin (PT0495R) rabbit antibody
Rat colon was stained with anti-Vimentin (PT0495R) rabbit antibody
Human colon was stained with anti-Vimentin (PT0495R) rabbit antibody
Human colon was stained with anti-Vimentin (PT0495R) rabbit antibody
Mouse colon was stained with anti-Vimentin (PT0495R) rabbit antibody
Mouse colon was stained with anti-Vimentin (PT0495R) rabbit antibody
For Research Use Only. Not for use in diagnostic procedures.