Smad2 (phospho Ser250) (PT0058R) PT® Rabbit mAb

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation

Product Overview

Entrez GenelD

4087

Clone#

PT0058R

Host / Isotype

Rabbit / IgG, Kappa

Species Reactivity

Human, Mouse, Rat

Formulation

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage

-15°C to -25°C / 1 year (Do not store below -25°C)

Product Applications

WB (Western Blot)

1/1000 - 1/5000

IHC_P(Immunohistochemistry)

1/100 - 1/500

ELISA

1/5000 - 1/20000

Product Image

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Smad2 (phospho Ser250) (PT0058R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 treated with TPA for 48 hours Lane 2: Hela Lane 3: H9C2 Lane 4: RAW264.7 Predicted band size: 58kDa Observed band size: 58kDa

For Research Use Only. Not for use in diagnostic procedures.