Rpb1 (PT0570R) PT® Rabbit mAb
Item Information
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Description
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008],
Product Overview
Entrez GenelD
5430
Clone#
PT0570R
Host / Isotype
Rabbit / IgG, Kappa
Species Reactivity
Human, Mouse, Rat
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage
-15°C to -25°C / 1 year (Do not store below -25°C)
Product Applications
WB (Western Blot)
1/2000 - 1/10000
IHC_P(Immunohistochemistry)
1/50 - 1/200
ELISA
1/5000 - 1/20000
Product Image
Human colon was stained with anti-Rpb1 (PT0570R) rabbit antibody
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Rpb1 (PT0570R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Lane 1: A549
Lane 2: Mouse lung
Lane 3: Rat lung
Predicted band size: 192kDa
Observed band size: 250kDa
Various whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-Rpb1 (PT0570R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Lane 1: Hela
Predicted band size: 192kDa
Observed band size: 250kDa
For Research Use Only. Not for use in diagnostic procedures.