Osteopontin (PT0442R) PT® Rabbit mAb

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Description

secreted phosphoprotein 1(SPP1) Homo sapiens    The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],

Product Overview
Entrez GenelD
6696
Clone#
PT0442R
Host / Isotype
Rabbit / IgG, Kappa
Species Reactivity
Human, Mouse, Rat
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage
-15°C to -25°C / 1 year (Do not store below -25°C)
Product Applications
WB (Western Blot)
1/2000 - 1/10000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/5000 - 1/20000
Product Image
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Osteopontin (PT0442R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Lane 1: A549
Lane 2: HepG2

Predicted band size: 35kDa
Observed band size: 60kDa
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Osteopontin (PT0442R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: HepG2 Predicted band size: 35kDa Observed band size: 60kDa
Human liver was stained with anti-Osteopontin (PT0442R) rabbit antibody
Human liver was stained with anti-Osteopontin (PT0442R) rabbit antibody
For Research Use Only. Not for use in diagnostic procedures.