Nucleolin (PT0100R) PT® Rabbit mAb

Item Information
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Description

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008],

Product Overview
Entrez GenelD
4691
Clone#
PT0100R
Host / Isotype
Rabbit / IgG, Kappa
Species Reactivity
Human, Mouse
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage
-15°C to -25°C / 1 year (Do not store below -25°C)
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/5000 - 1/20000
Product Image
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Nucleolin (PT0100R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Lane 1: A549
Lane 2: HepG2

Predicted band size: 77kDa
Observed band size: 110kDa
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Nucleolin (PT0100R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: A549 Lane 2: HepG2 Predicted band size: 77kDa Observed band size: 110kDa
For Research Use Only. Not for use in diagnostic procedures.