MLKL (PT0646R) PT® Rabbit mAb

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Description

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015],

Product Overview
Entrez GenelD
197259
Clone#
PT0646R
Host / Isotype
Rabbit / IgG, Kappa
Species Reactivity
Human, Mouse, Rat
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage
-15°C to -25°C / 1 year (Do not store below -25°C)
Product Applications
WB (Western Blot)
1/2000 - 1/10000
IHC_P(Immunohistochemistry)
1/1000 - 1/4000
ELISA
1/5000 - 1/20000
Product Image
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MLKL (PT0646R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Lane 1: Hela
Lane 2: U-251 MG
Lane 3: U-14
Lane 4: Rat womb

Predicted band size: 55kDa
Observed band size: 55kDa
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-MLKL (PT0646R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: U-251 MG Lane 3: U-14 Lane 4: Rat womb Predicted band size: 55kDa Observed band size: 55kDa
Humantonsil was stained with anti-MLKL (PT0646R) rabbit antibody
Humantonsil was stained with anti-MLKL (PT0646R) rabbit antibody
For Research Use Only. Not for use in diagnostic procedures.