LATS1 (PT0609R) PT® Rabbit mAb

Item Information

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Price

Description

The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatmen

Product Overview

Entrez GenelD

9113

Clone#

PT0609R

Host / Isotype

Rabbit / IgG, Kappa

Species Reactivity

Human, Mouse, Rat

Formulation

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage

-15°C to -25°C / 1 year (Do not store below -25°C)

Product Applications

WB (Western Blot)

1/2000 - 1/10000

ELISA

1/5000 - 1/20000

Product Image

Various whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-LATS1 (PT0609R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: C2C12 Lane 2: PC-12 Predicted band size: 127kDa Observed band size: 140kDa

Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LATS1 (PT0609R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: HT-1080 Predicted band size: 127kDa Observed band size: 140kDa

For Research Use Only. Not for use in diagnostic procedures.