Histone H2B (PT0420R) PT® Rabbit mAb

Item Information
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Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Two transcripts that encode the same protein have been identified for this gene, which is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

Product Overview
Entrez GenelD
3017, 3017, 8345, 85236, 8340, 8342, 8341, 440689
Clone#
PT0420R
Host / Isotype
Rabbit / IgG, Kappa
Species Reactivity
Human, Mouse, Rat
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage
-15°C to -25°C / 1 year (Do not store below -25°C)
Product Applications
WB (Western Blot)
1/20000 - 1/50000
IHC_P(Immunohistochemistry)
1/2000 - 1/10000
ELISA
1/5000 - 1/20000
Product Image
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H2B (PT0420R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Lane 1: K562
Lane 2: Hela
Lane 3: C6
Lane 4: 3T3-L1 

Predicted band size: 14kDa
Observed band size: 14kDa
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Histone H2B (PT0420R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: Hela Lane 3: C6 Lane 4: 3T3-L1 Predicted band size: 14kDa Observed band size: 14kDa
For Research Use Only. Not for use in diagnostic procedures.