His Tag (PT0481R) PT® Rabbit mAb
Item Information
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Description
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Product Overview
Clone#
PT0481R
Host / Isotype
Rabbit / IgG, Kappa
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage
-15°C to -25°C / 1 year (Do not store below -25°C)
Product Applications
WB (Western Blot)
1/2000 - 1/10000
ELISA
1/5000 - 1/20000
Product Image
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-His Tag (PT0481R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Lane 1: Flag-His-MBP
Lane 2: Myc-His-MBP
Lane 3: HA-His-MBP
Predicted band size: 50kDa
Observed band size: 55kDa
For Research Use Only. Not for use in diagnostic procedures.