ERK1/2 (PT0510R) PT® Rabbit mAb

Item Information
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Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008],

Product Overview
Entrez GenelD
1.000178763
Clone#
PT0510R
Host / Isotype
Rabbit / IgG, Kappa
Species Reactivity
Human, Mouse, Rat
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage
-15°C to -25°C / 1 year (Do not store below -25°C)
Product Applications
WB (Western Blot)
1/2000 - 1/10000
IHC_P(Immunohistochemistry)
1/2000 - 1/10000
ELISA
1/5000 - 1/20000
Product Image
Human breast carcinoma was stained with anti-ERK1/2 (PT0510R) rabbit antibody
Human breast carcinoma was stained with anti-ERK1/2 (PT0510R) rabbit antibody
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ERK1/2 (PT0510R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Lane 1: 3T3-L1
Lane 2: Hela 
Lane 3: C6
Lane 4: K562

Predicted band size: 43,41kDa
Observed band size: 43,41kDa
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ERK1/2 (PT0510R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: 3T3-L1 Lane 2: Hela Lane 3: C6 Lane 4: K562 Predicted band size: 43,41kDa Observed band size: 43,41kDa
For Research Use Only. Not for use in diagnostic procedures.