Erk 1/2 (Phospho Thr202/Tyr204) (PT0643R) PT® Rabbit mAb
Item Information
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Size
Price
Description
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008],
Product Overview
Entrez GenelD
5594, 5595
Clone#
PT0643R
Host / Isotype
Rabbit / IgG, Kappa
Species Reactivity
Human, Mouse, Rat
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage
-15°C to -25°C / 1 year (Do not store below -25°C)
Product Applications
WB (Western Blot)
1/2000 - 1/10000
ELISA
1/5000 - 1/20000
Product Image
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Erk1/2(phospho Thr202/Tyr204) (PT0643R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Lane 1: Hela
Lane 2: Hela was starved of serum for 24 hours and then treated with 20% fetal bovine serum for 15 minutes, followed by Calyculin A treatment for 15 minutes
Predicted band size: 44,42kDa
Observed band size: 44,42kDa
For Research Use Only. Not for use in diagnostic procedures.