eIF2α (PT0480R) PT® Rabbit mAb
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Description
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010],
Product Overview
Entrez GenelD
1965
Clone#
PT0480R
Host / Isotype
Rabbit / IgG, Kappa
Species Reactivity
Human, Mouse, Rat
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage
-15°C to -25°C / 1 year (Do not store below -25°C)
Product Applications
WB (Western Blot)
1/2000 - 1/10000
IHC_P(Immunohistochemistry)
1/1000 - 1/5000
ELISA
1/5000 - 1/20000
Product Image
Human kidney was stained with anti-eIF2α (PT0480R) rabbit antibody
Rat kidney was stained with anti-eIF2α (PT0480R) rabbit antibody
Human breast was stained with anti-eIF2α (PT0480R) rabbit antibody
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-eIF2α (PT0480R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Lane 1: HepG2
Lane 2: RAW264.7
Lane 3: Hela
Lane 4: C6
Predicted band size: 36kDa
Observed band size: 36kDa
For Research Use Only. Not for use in diagnostic procedures.