DDX5 (PT0429R) PT® Rabbit mAb

Item Information
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Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016],

Product Overview
Entrez GenelD
1655
Clone#
PT0429R
Host / Isotype
Rabbit / IgG, Kappa
Species Reactivity
Human, Mouse, Rat
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage
-15°C to -25°C / 1 year (Do not store below -25°C)
Product Applications
WB (Western Blot)
1/1000 - 1/5000
IHC_P(Immunohistochemistry)
1/1000 - 1/5000
ELISA
1/5000 - 1/20000
Product Image
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-DDX5 (PT0429R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Lane 1: Hela
Lane 2: C6
Lane 3: 3T3-L1
Lane 4: U-87 MG

Predicted band size: 69kDa
Observed band size: 69kDa
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-DDX5 (PT0429R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: C6 Lane 3: 3T3-L1 Lane 4: U-87 MG Predicted band size: 69kDa Observed band size: 69kDa
For Research Use Only. Not for use in diagnostic procedures.