ATG5 (PT0514R) PT® Rabbit mAb

Item Information
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Description

The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2015],

Product Overview
Entrez GenelD
9474
Clone#
PT0514R
Host / Isotype
Rabbit / IgG, Kappa
Species Reactivity
Human, Mouse, Rat
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage
-15°C to -25°C / 1 year (Do not store below -25°C)
Product Applications
WB (Western Blot)
1/2000 - 1/10000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/5000 - 1/20000
Product Image
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ATG5 (PT0514R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Lane 1: U-87 MG
Lane 2: Hela
Lane 3: C6
Lane 4: 3T3-L1 

Predicted band size: 33kDa
Observed band size: 55kDa
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ATG5 (PT0514R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: U-87 MG Lane 2: Hela Lane 3: C6 Lane 4: 3T3-L1 Predicted band size: 33kDa Observed band size: 55kDa
Human colon was stained with anti-ATG5 (PT0514R) rabbit antibody
Human colon was stained with anti-ATG5 (PT0514R) rabbit antibody
For Research Use Only. Not for use in diagnostic procedures.