ALPP (PT0484R) PT® Rabbit mAb

Item Information
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Description

The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015],

Product Overview
Entrez GenelD
250
Clone#
PT0484R
Host / Isotype
Rabbit / IgG, Kappa
Species Reactivity
Human, Mouse, Rat
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage
-15°C to -25°C / 1 year (Do not store below -25°C)
Product Applications
WB (Western Blot)
1/2000 - 1/10000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/5000 - 1/20000
Product Image
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ALPP (PT0484R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Lane 1: Hela
Lane 2: JAR
Lane 3: Rat small intestine 

Predicted band size: 58kDa
Observed band size: 70kDa
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-ALPP (PT0484R) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: JAR Lane 3: Rat small intestine Predicted band size: 58kDa Observed band size: 70kDa
Human placenta was stained with anti-ALPP (PT0484R) rabbit antibody
Human placenta was stained with anti-ALPP (PT0484R) rabbit antibody
For Research Use Only. Not for use in diagnostic procedures.