ZFP91 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the zinc finger family of proteins. The gene product contains C2H2-type domains, which are the classical zinc finger domains found in numerous nucleic acid-binding proteins. This protein functions as a regulator of the non-canonical NF-kappaB pathway in lymphotoxin-beta receptor signaling. Alternative splicing results in multiple transcript variants. A read-through transcript variant composed of ZFP91 and the downstream CNTF gene sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. A ZFP91-related pseudogene has also been identified on chromosome 2.
Product Overview
Entrez GenelD
80829
Aliases
PZF; DMS-8; DSM-8; FKSG11; ZFP-91; ZNF757
Clone#
7G11H2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ZFP91 (AA: 162-304) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/100 - 1/500
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Pathol Oncol Res. 2014 Apr;20(2):453-9.
2.Biochem Biophys Res Commun. 2010 Oct 1;400(4):581-6.
2.Biochem Biophys Res Commun. 2010 Oct 1;400(4):581-6.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ZFP91 mAb against human ZFP91 (AA: 162-304) recombinant protein. (Expected MW is 43 kDa)
Western Blot
Figure 3:Western blot analysis using ZFP91 mAb against HEK293 (1) and ZFP91 (AA: 162-304)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using ZFP91 mouse mAb against Jurkat (1), A431 (2), HepG2 (3), HEK293 (4), and A549 (5) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using ZFP91 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis
Figure 6:Immunofluorescence analysis of Hela cells using ZFP91 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 7:Flow cytometric analysis of Hela cells using ZFP91 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using ZFP91 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ZFP91 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.