ZFP42 Primary Antibody
Item Information
Catalog #
Size
Price
Description
ZFP42 involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency. Required for efficient elongation of TSIX, a non-coding RNA antisense to XIST. Binds DXPas34 enhancer within the TSIX promoter.
Product Overview
Entrez GenelD
132625
Aliases
REX1; ZNF754
Clone#
5E11E7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ZFP42 (AA: 249-310) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Stem Cell Res. 2011 Jul;7(1):1-16.
2. J Cell Physiol. 2010 Jul;224(1):17-27.
2. J Cell Physiol. 2010 Jul;224(1):17-27.
Product Image
Western Blot
Figure 1: Western blot analysis using ZFP42 mAb against human ZFP42 recombinant protein. (Expected MW is 32.7 kDa)
Western Blot
Figure 2: Western blot analysis using ZFP42 mAb against HEK293 (1) and ZFP42 (AA: 249-310)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using ZFP42 mouse mAb against Jurkat (1), HEK293 (2), Raji (3) and PC-3 (4) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of HEK293 cells using ZFP42 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ZFP42 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues using ZFP42 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.