YAP1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction.
Product Overview
Entrez GenelD
10413
Aliases
YAP; YKI; YAP2; YAP65
Clone#
1A12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human YAP1 expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Genes Dev. 2009 Dec 1;23(23):2729-41.
2. Nat Cell Biol. 2009 Dec;11(12):1444-50.
2. Nat Cell Biol. 2009 Dec;11(12):1444-50.
Product Image
Western Blot
Figure 1: Western blot analysis using YAP1 mAb against human YAP1 (AA: 250-447) recombinant protein. (Expected MW is 54.4 kDa)
Western Blot
Figure 2: Western blot analysis using YAP1 mouse mAb against Hela (1), C6 (2) and Cos7 (3) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using YAP1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using YAP1 mouse mAb with DAB staining.
Flow cytometric
Figure 5: Flow cytometric analysis of Hela cells using YAP1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.