XRCC6 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.
Product Overview
Immunogen
Purified recombinant fragment of human XRCC6 (AA: 6-214) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
4°C; -20°C for long term storage
Product Image
Western Blot

Figure 1: Western blot analysis using XRCC6 mAb against human XRCC6 (AA: 6-214) recombinant protein. (Expected MW is 49.7 kDa)
Western Blot

Figure 2: Western blot analysis using XRCC6 mAb against HEK293 (1) and XRCC6 (AA: 6-214)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot

Figure 3: Western blot analysis using XRCC6 mouse mAb against Hela (1), PC-2 (2), A549 (3), A431 (4), HepG2 (5), K562 (6) cell lysate.
Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of MCF-7 cells using XRCC6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric

Figure 5: Flow cytometric analysis of A431 cells using XRCC6 mouse mAb (green) and negative control (red).
Immunohistochemical analysis

Figure 6: Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using XRCC6 mouse mAb with DAB staining.
Immunohistochemical analysis

Figure 7: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using XRCC6 mouse mAb with DAB staining.
Elisa

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.