XRCC5 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesKU80; KUB2; Ku86; NFIV; KARP1; KARP-1; FLJ39089
ProductOrderX
Clone#5C5
Entrez GenelD7520
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human XRCC5 expressed in E. Coli.
MW86kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
61-340
Catalog#: 30237
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Images
Western Blot
Figure 1: Western blot analysis using XRCC5 mouse mAb against Hela (1), MCF-7 (2), A549 (3) and NIH/3T3 (4) cell lysate.
Figure 1: Western blot analysis using XRCC5 mouse mAb against Hela (1), MCF-7 (2), A549 (3) and NIH/3T3 (4) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human tonsil tissues (left) and human colon cancer tissues (right) using XRCC5 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded human tonsil tissues (left) and human colon cancer tissues (right) using XRCC5 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of Hela cells using XRCC5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Immunofluorescence analysis of Hela cells using XRCC5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of Hela cells using XRCC5 mouse mAb (green) and negative control (purple).
Figure 4: Flow cytometric analysis of Hela cells using XRCC5 mouse mAb (green) and negative control (purple).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Product Overview
Description

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

References (references)
References (references)1. Breast Cancer Res. 2009;11(6):R83.
2. Biochem Biophys Res Commun. 2009 Dec 18;390(3):738-42.