Mouse Monoclonal Antibody to XRCC1
Item Information
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Description
The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
Product Overview
Entrez GenelD
7515
Aliases
RCC; SCAR26
Clone#
7B4C12
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human XRCC1 (AA:1-150) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Zhonghua Yi Xue Za Zhi. 2021 Mar 23;101(11):759-765.2,Meta-Analysis Biomed Res Int. 2020 Oct 22;2020:3520764.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using XRCC1 mAb against human XRCC1 (AA:1-150) recombinant protein. (Expected MW is 42.5 kDa)
Western Blot
Figure 3:Western blot analysis using XRCC1 mAb against HEK293-6e (1) and human XRCC1 (AA:1-150)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using XRCC1 mouse mAb against Hela (1), Jurkat (2),k562 (3),SK-OV-3 (4), and COS-7 (5) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Jurkat cells using XRCC1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded bladder Cancer tissues using Jurkat mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using Jurkat mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using Jurkat mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.