WNT3A Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesN
ProductOrderW
Clone#1E6G4
Entrez GenelD89780
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG2b
ImmunogenPurified recombinant fragment of human WNT3A (AA: 170-352) expressed in E. Coli.
MW39.4kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
170-352
Catalog#: 30902
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using WNT3A mAb against human WNT3A (AA: 170-352) recombinant protein. (Expected MW is 47.1 kDa)
Figure 2:Western blot analysis using WNT3A mAb against human WNT3A (AA: 170-352) recombinant protein. (Expected MW is 47.1 kDa)
Western Blot
Figure 3:Western blot analysis using WNT3A mAb against HEK293 (1) and WNT3A (AA: 170-352)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using WNT3A mAb against HEK293 (1) and WNT3A (AA: 170-352)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using WNT3A mouse mAb against A549 (1), HepG2 (2), and A431 (3) cell lysate.
Figure 4:Western blot analysis using WNT3A mouse mAb against A549 (1), HepG2 (2), and A431 (3) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of HeLa cells using WNT3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of HeLa cells using WNT3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of HeLa cells using WNT3A mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of HeLa cells using WNT3A mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using WNT3A mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using WNT3A mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using WNT3A mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using WNT3A mouse mAb with DAB staining.
Product Overview
DescriptionThe WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region.
References (references)
References (references)1.Mol Cell Neurosci. 2013 May;54:44-57.
2.J Dermatol Sci. 2011 Dec;64(3):199-209.