WNT10B Primary Antibody
Item Information
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Price
Description
WNT10B: wingless-type MMTV integration site family, member 10B. The WNT family consists of structurally related secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT10B is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is ikely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10B protein at the amino acid level. The WNT10B gene is clustered with another family member, WNT1, in the chromosome 12q13 region.
Product Overview
Entrez GenelD
7480
Aliases
SHFM6; WNT-12
Clone#
5A7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human WNT10B expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Oncogene. 1997 Mar 13;14(10):1249-53.
2. Int J Oncol. 2001 Dec;19(6):1187-92
2. Int J Oncol. 2001 Dec;19(6):1187-92
Product Image
Western Blot
Figure 1: Western blot analysis using WNT10B mouse mAb against Hela cell lysate (1).
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human normal stomach (A), normal liver (B), normal kidney (C) and rectum cancer tissues (D) using WNT10B mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Confocal Immunofluorescence analysis of PANC-1 cells using WNT10B mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
For Research Use Only. Not for use in diagnostic procedures.