WAS Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesTHC; IMD2; SCNX; THC1; WASP
ProductOrderW
Clone#7B10E4
Entrez GenelD7454
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG2a
ImmunogenPurified recombinant fragment of human WAS (AA: 57-170) expressed in E. Coli.
MW53kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
57-170
Catalog#: 30716
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Images
Western Blot
Figure 1: Western blot analysis using WAS mAb against human WAS (AA: 57-170) recombinant protein. (Expected MW is 39 kDa)
Figure 1: Western blot analysis using WAS mAb against human WAS (AA: 57-170) recombinant protein. (Expected MW is 39 kDa)
Western Blot
Figure 2: Western blot analysis using WAS mAb against HEK293 (1) and WAS (AA: 57-170)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using WAS mAb against HEK293 (1) and WAS (AA: 57-170)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using WAS mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of Hela cells using WAS mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using WAS mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using WAS mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using WAS mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using WAS mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known.

References (references)
References (references)1. Mol Cell Biol. 2012 Aug;32(15):3153-63.
2. Dis Markers. 2010;29(3-4):157-75.