Mouse Monoclonal Antibody to VCP
Item Information
Catalog #
Size
Price
Description
This protein forms a homohexameric complex that interacts with a variety of cofactors and extracts ubiquitinated proteins from lipid membranes or protein complexes. Mutations in this gene cause IBMPFD (inclusion body myopathy with paget disease of bone and frontotemporal dementia), ALS (amyotrophic lateral sclerosis) and Charcot-Marie-Tooth disease in human patients.
Product Overview
Entrez GenelD
7415
Aliases
p97; TERA; CDC48; FTDALS6
Clone#
8E4A4
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human VCP (AA:707-806) expressed in Mammalian system
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,mBio. 2020 Apr 14;11(2):e00467-20. 2,Nat Commun. 2021 Jan 29;12(1):713.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using VCP mAb against human VCP (AA:707-806) recombinant protein. (Expected MW is 42 kDa)
Western Blot
Figure 3:Western blot analysis using VCP mouse mAb against Hela (1), A549 (2), NIH/3T3 (3), Raw264.7 (4), SH-SY5Y (5), MCF-7 (6), and A431 (7) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of MOLT4 cells using VCP mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of HL-60 cells using VCP mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of THP-1 cells using VCP mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 7:Immunofluorescence analysis of Hela cells using VCP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.