Mouse Monoclonal Antibody to VASP
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/2330389fb80dab8fba5e00d297207da0b830e76fb9182bcf9969f7fbd618b1cb.jpg?v=1666705983)
![Figure 2:Western blot analysis using VASP mAb against human VASP (AA: 1-380) recombinant protein. (Expected MW is 42.6 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/34aa628e9f489a1f0ba888aa76628c5e1b4a6ef63922e84b42d97233c6b24e9c.jpg?v=1666705983)
![Figure 3:Western blot analysis using VASP mAb against HEK293-6e (1) and VASP (AA: 1-380)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a910a57a645ad297feb63e12d9dc50086b8fb59458ee75d90964f7bfbe209d70.jpg?v=1666705983)
![Figure 4:Western blot analysis using VASP mouse mAb against THP-1 (1), Hela (2), HepG2 (3), HT-29 (4), and A549 (5) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/dad53afd6fe49c7e9b1dd1469dc9e78a92f4f3d277924009b95a5f63673b5d3f.jpg?v=1666705983)
![Figure 5:Flow cytometric analysis of THP-1 cells using VASP mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/d5ac798f6fabb7f34e941197350f8af87db887b7ff8977d02726933b852ab02e.jpg?v=1666705983)
![Figure 6:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using VASP mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/216ebdad9146549cb7c03f9ac9656e878f1659b023ab464b7483b16bf43e396d.jpg?v=1666705983)
![Figure 7:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using VASP mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/9cc889e6212ea4a395b9c9c6e5736be8271b1db6b4b3db00b4f1ede5277ac79a.jpg?v=1666705983)