Mouse Monoclonal Antibody to VASP
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/4c3109e2b61a8ec91e6f3908f9cc888325bbf683255ce8915560f86224789ea7.jpg?v=1666705856)
![Figure 2:Western blot analysis using VASP mAb against human VASP (AA: 1-380) recombinant protein. (Expected MW is 42.6 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ab9f8f24d6e8d5a06c98945339c8dd2b8a32445e0432a9b9511b6e3064e0272d.jpg?v=1666705856)
![Figure 3:Western blot analysis using VASP mAb against HEK293-6e (1) and VASP (AA: 1-380)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/2866718360637f7ca899cd9899e7d6b17a65d2ee865d28a27421bbb905481a07.jpg?v=1666705856)
![Figure 4:Western blot analysis using VASP mouse mAb against THP-1 (1), HepG2 (2), Hela (3), HT-29 (4), MCF-7 (5), A549 (6), and COS-7 (7) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e45f2ed9b706a87094edf22f8bbcc256de8551d8d73ac8903be7753d3e2bba47.jpg?v=1666705856)
![Figure 5:Flow cytometric analysis of MOLT4 cells using VASP mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/cad77810434f2dbaea788924197a44141922376432ef324172f0df2f1ea4fb01.jpg?v=1666705856)
![Figure 6:Flow cytometric analysis of THP-1 cells using VASP mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ebbe54c51b708ba36627c6ec102ddbae2f97028610ac5c3048ca3813dfa3bccb.jpg?v=1666705856)
![Figure 7:Flow cytometric analysis of HL-60 cells using VASP mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/560549598ff6b6cfdfb983159781a92d8e885abfee22f24d2a4caa7e2b2a9c27.jpg?v=1666705856)
![Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using VASP mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/100ed26aee2205da4b2eab8add00c97a22f987ab4656f4ec771d127dc257741a.jpg?v=1666705856)
![Figure 9:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using VASP mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/b35ef50a44c0dbd155c29a982e15274d3280381f6c4a025b692fd7d5f7af47d2.jpg?v=1666705856)
![Figure 10:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using VASP mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/23e516cc2de987cf2e11e5fd11690db0a77094788faac1527b4334fb30a7b366.jpg?v=1666705856)
![Figure 11:Immunofluorescence analysis of Hela cells using VASP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e69d0340a35fe4dbcba0b01fa992ba88896c07c1db2cf529980bf56d084c38d6.jpg?v=1666705858)