Mouse Monoclonal Antibody to VASP

Item Information
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Description

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]

Product Overview
Entrez GenelD
7408
Clone#
6B10A1
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human VASP (AA: 1-380) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Proc Natl Acad Sci U S A. 2020 Nov 24;117(47):29684-29690.2,PLoS Pathog. 2020 Sep 30;16(9):e1008879.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using VASP mAb against human VASP (AA: 1-380) recombinant protein. (Expected MW is 42.6 kDa)
Figure 2:Western blot analysis using VASP mAb against human VASP (AA: 1-380) recombinant protein. (Expected MW is 42.6 kDa)
Western Blot
Figure 3:Western blot analysis using VASP mAb against HEK293-6e (1) and VASP (AA: 1-380)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using VASP mAb against HEK293-6e (1) and VASP (AA: 1-380)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using VASP mouse mAb against THP-1 (1), HepG2 (2), Hela (3), HT-29 (4), MCF-7 (5), A549 (6), and COS-7 (7) cell lysate.
Figure 4:Western blot analysis using VASP mouse mAb against THP-1 (1), HepG2 (2), Hela (3), HT-29 (4), MCF-7 (5), A549 (6), and COS-7 (7) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of MOLT4 cells using VASP mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of MOLT4 cells using VASP mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of THP-1 cells using VASP mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of THP-1 cells using VASP mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 7:Flow cytometric analysis of HL-60 cells using VASP mouse mAb (green) and negative control (red).
Figure 7:Flow cytometric analysis of HL-60 cells using VASP mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using VASP mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using VASP mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using VASP mouse mAb with DAB staining.
Figure 9:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using VASP mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 10:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using VASP mouse mAb with DAB staining.
Figure 10:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using VASP mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 11:Immunofluorescence analysis of Hela cells using VASP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 11:Immunofluorescence analysis of Hela cells using VASP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.