Mouse Monoclonal Antibody to USP7
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Description
The protein encoded by this gene belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. This protein deubiquitinates target proteins such as p53 (a tumor suppressor protein) and WASH (essential for endosomal protein recycling), and regulates their activities by counteracting the opposing ubiquitin ligase activity of proteins such as HDM2 and TRIM27, involved in the respective process. Mutations in this gene have been implicated in a neurodevelopmental disorder. [provided by RefSeq, Mar 2016]
Product Overview
Immunogen
Purified recombinant fragment of human USP7 (AA:1-208) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Image
Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot

Figure 2:Western blot analysis using USP7 mAb against human USP7 (AA: 1-208) recombinant protein. (Expected MW is 27 kDa)
Western Blot

Figure 3:Western blot analysis using USP7 mAb against HEK293 (1) and human USP7 (AA:1-208 )-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot

Figure 4:Western blot analysis using *** mouse mAb against Hela (1), A431 (2), MCF-7 (3),Jurkat (4),K562 (5),HepG2 (6),A549 (7),HCT116 (8),HT-29 (9),SW480 (10),C6 (11),COS-7 (12),and NIH/3T3 (13) cell lysate.
Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using USP7 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis

Figure 6:Flow cytometric analysis of Hela cells using USP7 mouse mAb (green) and negative control (red).
Flow cytometric analysis

Figure 7:Flow cytometric analysis of HepG2 cells using USP7 mouse mAb (green) and negative control (red).
Flow cytometric analysis

Figure 8:Flow cytometric analysis of Jurkat cells using USP7 mouse mAb (green) and negative control (red).
Flow cytometric analysis

Figure 9:Flow cytometric analysis of K562 cells using USP7 mouse mAb (green) and negative control (red).
Immunohistochemical analysis

Figure 10:Immunohistochemical analysis of paraffin-embedded esophageal carcinoma tissues using USP7 mouse mAb with DAB staining.
Immunohistochemical analysis

Figure 11:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using USP7 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.