Mouse Monoclonal Antibody to USP7
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. This protein deubiquitinates target proteins such as p53 (a tumor suppressor protein) and WASH (essential for endosomal protein recycling), and regulates their activities by counteracting the opposing ubiquitin ligase activity of proteins such as HDM2 and TRIM27, involved in the respective process. Mutations in this gene have been implicated in a neurodevelopmental disorder. [provided by RefSeq, Mar 2016]
Product Overview
Entrez GenelD
7874
Aliases
TEF1; HAUSP; HAFOUS
Clone#
6A3F3
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human USP7 (AA:1-208) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Theranostics. 2020 Jul 23;10(20):9332-9347. 2,J Virol. 2020 Feb 14;94(5):e01638-19.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using USP7 mAb against human USP7 (AA: 1-208) recombinant protein. (Expected MW is 27 kDa)
Western Blot
Figure 3:Western blot analysis using USP7 mAb against HEK293 (1) and human USP7 (AA:1-208 )-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using *** mouse mAb against Hela (1), A431 (2), MCF-7 (3),Jurkat (4),K562 (5),HepG2 (6),A549 (7),HCT116 (8),HT-29 (9),SW480 (10),C6 (11),COS-7 (12),and NIH/3T3 (13) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using USP7 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 6:Flow cytometric analysis of Hela cells using USP7 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 7:Flow cytometric analysis of HepG2 cells using USP7 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 8:Flow cytometric analysis of Jurkat cells using USP7 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 9:Flow cytometric analysis of K562 cells using USP7 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 10:Immunohistochemical analysis of paraffin-embedded esophageal carcinoma tissues using USP7 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 11:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using USP7 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.